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Perfuse
the animal transcardially with Buffer, followed by fix. |
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1.
Prepre the animal for perfusion by having all the necessary solutions
and tools ready. > find
out more |
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2.
Expose the heart by making key incisions. > find
out more |
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3.
Perfuse the animal with ice-cold
PBS, followed by ice-cold 4% paraformaldehyde in 0.1 M phosphate
buffer (PB, pH 7.4). > find
out more |
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