1. Make Animal Sleep
2. Perfuse Animal
3. Remove Brain
4. Fix Brain
 
 
 
 
 
 

   
  Immerse the removed brain in the fix for 24-72 hours.
   
  1. Immerse the removed brain into freshly made fix (4% para-formaldehyde, PBS). > find out more
   
  2. Wait a day or two depending on the size and the volume of the brain and/or hippocampus. > find out more