1. Prepare Hippo. Sections
2. PrimaryAB Incubation
3. SecondaryAB Incubation
4. Mount Hippo. Sections
 
 
 
 
 
 
   
  Initial preparation of the hippo. sections just prior to PrimaryAB Incubation depends on the PrimaryAB itself.
   
  1. Carefully Select (i.e. random sampling) the hippo. sections to be used for "the cooking" and place them in 24 well plate. > find out more
   
  2. Wash the selected hippo. sections (sitting in 24 well plate) with PBS 3 times, 5 minutes each. > find out more
   
  3. Determine which preparation method to use depending on the PrimaryAB and carry it out. > find out more