1. Prepare Hippo. Sections
2. PrimaryAB Incubation
3. SecondaryAB Incubation
4. Mount Hippo. Sections
 
 
 
 
 
 
   
  PrimaryAB Incubation results in binding of your PrimaryAB to the targeted AG.
   
  1. Prepare your PrimaryAB by determining the dilution ratio and dilute the PrimaryAB in (0.3% Triton-X, PBS). > find out more
   
  2. Remove the PBS from the 24 well plate and replace with prepared PrimaryAB solution (making sure that the hippo. sections are well covered). > find out more
   
  3. Place the 24 well plate in the cold room (4°C) for 24 to 72 hours, while shaking gently. > find out more