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SecondaryAB
Incubation results in binding of your SecondaryAB to the PrimaryAB. |
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1.
Determine which SecondaryAB to use (depends on the PrimaryAB). >
find
out more |
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2.
Determine the dilution ratio of SecondaryAB and dilute the SecondaryAB
in (0.3% Triton-X, PBS). > find
out more |
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3.
Remove the PrimaryAB solution from the 24 well plate, wash 3 times
with PBS and incubate with SecondaryAB solution for 2-4 hours.
> find
out more |
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