1. Prepare Hippo. Sections
2. PrimaryAB Incubation
3. SecondaryAB Incubation
4. Mount Hippo. Sections
 
 
 
 
 
 
   
  Finally, mounting your labelled hippo. sections onto glass slides.
   
  1. Wash the hippo. sections (sitting in 24 well plate) with PBS 3 times, 5 minutes each. > find out more
   
  2. Place labelled hippo. sections in large petri dish filled with distilled H2O and let it sit for 2-3 minutes before rolling them onto glass slides. > find out more
   
  3. Dry the glass slide until moisture is barely visible and apply a few drops of PermaFluor before covering with cover slip. > find out more